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ASCP ASCP-MLT Exam Syllabus Topics:

SectionWeightObjectives
Topic 1: Immunology5–10%- Serologic procedures and immune diseases
- Principles of immunology
Topic 2: Laboratory Operations5–10%- Quality assessment and safety
- Instrumentation and laboratory mathematics
Topic 3: Urinalysis and Other Body Fluids5–10%- Physical and chemical testing
- Microscopic analysis and disease correlation
Topic 4: Microbiology15–20%- Virology and parasitology testing
- Bacteriology and mycology procedures
Topic 5: Blood Banking15–20%- Blood group systems and immunology
- Blood products and components
- Transfusion practice and testing
Topic 6: Chemistry20–25%- Electrolytes, acid-base, and toxicology
- Carbohydrates, lipids, and enzymes
Topic 7: Hematology20–25%- Laboratory testing and hemostasis
- Physiology and disease states

ASCP MEDICAL LABORATORY TECHNICIAN - MLT(ASCP) Sample Questions:

1. Twelve weeks after onset of the disease, patients with uncomplicated acute hepatitis B usually will demonstrate which of the following in their serum?

A) Anti-HIV
B) HBsAg
C) Anti-HBe
D) Anti-HTLV


2. Synovial fluid is actually quite viscous and does not have the same consistency as plasma. The reason for its thicker consistency is to provide cushion and reduce friction between the articular cartilage of synovial joints during movement.
Urinalysis & Other Body Fluids
All of the statements below about synovial fluid are true EXCEPT:

A) joint fluid resembles plasma in viscosity
B) synovial fluid is present only in movable joints
C) arthrocentesis is performed as a means to evaluate arthritis and other joint disorders
D) joint fluid has approximately the same glucose as plasma


3. Alkaline phosphatase, or ALP, is present in kidneys, liver, intestines, bone, and the placenta. The liver makes the largest amount of ALP. Some of the conditions associated with increased levels of ALP include: damaged liver cells, rapid bone growth (during puberty), bone diseases, or a disease that affects how much calcium is in the blood (hyperparathyroidism), and vitamin D deficiency.
Chemistry
Isoenzymes of alkaline phosphatase occur in:

A) Brain, kidney, intestines, bone
B) Kidney, bone, intestines, liver
C) Liver, brain, spleen, intestines
D) Bone, brain, liver, pancreas


4. Considering the reactions given in this case study, Yersinia enterocolitica would be the best choice.
Most strains of Plesiomonas shigelloides, Escherichia coli, and Pasteurella multocida are urease negative.
Also, Plesiomonas shigelloides demonstrates a K/A reaction on a TSI slant.
The stool specimen from a patient admitted to the hospital with symptoms of appendicitis has a culture with the following characteristics:
Gram-negative bacillus
Catalase positive
Urease positive
Weakly fermentative
TSI Slant is A/A
These reactions suggest that the organism is MOST likely:

A) Pasteurella multocida
B) Plesiomonas shigelloides
C) Escherichia coli
D) Yersinia enterocolitica


5. When an antigen comes in contact with the skin, the antigen is processed by cells in the epidermis and come in contact with T lymphocytes. T lymphocytes recognize the antigen as foreign and circulate through the bloodstream back to the epidermis and produce an inflammatory response to eliminate the antigen, but this immune response can produce a characteristic rash in the skin called contact dermititis.
Contact dermatitis is mediated by:

A) Chemokines
B) B lymphocytes
C) T lymphocytes
D) Polymorphonuclear cells
E) Macrophages


Solutions:

Question # 1
Answer: C
Question # 2
Answer: A
Question # 3
Answer: B
Question # 4
Answer: D
Question # 5
Answer: C

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